Tudor Rose

Nova Pilbeam

Lady Jane Grey

Cedric Hardwicke

Earl of Warwick

John Mills

Lord Guilford Dudley

Felix Aylmer

Edward Seymour

Leslie Perrins

Thomas Seymour

Frank Cellier

Henry VIII

Desmond Tester

Edward VI

Gwen Ffrangcon Davies

Mary Tudor (as Gwen Ffrangcon-Davies)

Martita Hunt

Jane's Mother

Miles Malleson

Jane's Father

Sybil Thorndike

Ellen

Roy Emerton

Squire

C. V. France

Clergy at Execution

John Laurie

John Knox

John Singer

Boy

John Turnbull

Arundel

Peter Croft

Confidant of Thomas Seymour

Albert Davies

Barnaby Fitzpatrick

Shaun Desmond

Undetermined Role

Edward Dignon

Undetermined Role

Arthur Goullet

Sir John Gates

Henry Hallett

Undetermined Role

Eugene Leahy

Undetermined Role

Fewlass Llewellyn

Undetermined Role